| 摘要 |
FIELD: medicine; biotechnologies. ^ SUBSTANCE: way includes cultivation of fresh-water diatoms Synedra Acus in a photobioreactor on the medium containing an isotope 13C-containing component. The seaweed selected from a natural source is used for inoculation, or a biomass preliminary enriched by an isotope 13C. Cultivation is performed in the conditions excluding access of extraneous sources of carbon from the environment. In quality of isotope-containing reagent bicarbonate NaH13CO3 is used. Further biomass selection, its filtering, homogenising and extracting of the common lipids using an organic dissolvent is performed. Hydrolysis of the common lipids and the subsequent clearing of a target product by chromatography is carriedout. The way allows raising enrichment level with a stable isotope of received polynonsaturated fatty acids. Degree of enrichment by an isotope 13C of palmitoleic acid makes 60%, stearin acid - 18%, linolenic acid - 75%, eicosapentanoic acid - 64%. ^ EFFECT: rising of degree of enrichment by an isotope. ^ 6 cl, 2 dwg, 1 tbl, 2 ex |
