MICROARRAY-BASED GLOBAL CHROMATIN STRUCTURE MAPPING

摘要

Microarray-based methods for identifying positions of protein-DNA complexes on genomic DNA, including in particular human genomic DNA, are provided. The methods can be used to locate positioned nucleosomes, pre-initiation complexes, and other protein-DNA complexes on a global level, thereby permitting gene expression profiling based on microarray-based analysis of genomic DNA. Methods are provided to characterize and compare various cell types, including cancer cells. Also provided are methods for screening effects of test agents and pharmaceutical agents, including histone deacetylase inhibitors. Also provided are methods for identifying transcription start sites, transcriptionally active promoters, transcription factor binding sites, and transcription factors involved in the regulation of gene expression, including for microRNAs.