PRODUCTION OF FUSION PROTEINS BY CELL-FREE PROTEIN SYNTHESIS

摘要

The present invention relates to in vitro protein synthesis (IVPS) systems, particularly such systems using suppressor tRNAs and rare codon tRNAs to extend translation of an open reading frame into fusion protein elements, thereby generating fusion proteins in vitro. The invention also provides methods, compositions and kits using the IVPS systems of the invention, and proteins produced using the methods, compositions, kits and IVPS systems of the invention.