METAL MEDIATED FLUORESCENCE SUPERQUENCHING ASSAYS FOR DETECTION OF PHOSPHORYLATION AND DEPHOSPHORYLATION OF QUENCHER-LABELED SUBSTRATES
摘要
A method includes contacting an analyte; a phosphorylatable or dephosphorylatable substrate bound to a quencher; and a sensor that includes at least one fluorescent polymer complexed to one or more metal species; to form a composition; and determining the fluorescence of the composition, if any; wherein when the substrate is phosphorylated, an association occurs between the substrate and the sensor such that fluorescent superquenching occurs between the fluorescent polymer and the quencher.
申请公布号
WO2006102129(A3)
申请公布日期
2009.04.16
申请号
WO2006US09809
申请日期
2006.03.20
申请人
QTL BIOSYSTEMS LLC;RININSLAND, FRAUKE;STANKEWICZ, CASEY;WHITTEN, DAVID
发明人
RININSLAND, FRAUKE;STANKEWICZ, CASEY;WHITTEN, DAVID