METHOD OF PATTERNING PARTICLES ON AN ARBITRARY SUBSTRATE AND CONDUCTING A MICROFLUIDIC INVASION ASSAY

摘要

A method is provided for sequentially patterning different particle populations on spatially defined regions in microfluidic device. The microfluidic device has a channel and a plurality of access ports therein. Each access port has an input and an output communicating with the channel. The method includes the step of depositing a drop of a first suspension on the input of a first access port. The first suspension includes a plurality of particles. A drop of a second suspension is deposited on the input of a second access port. The second suspension includes a plurality of particles. The particles in the first and second suspensions settle onto and are patterned along corresponding spaced portions of the channel.