发明名称 |
Q-MELT FOR POLYMORPHISM DETECTION |
摘要 |
The invention provides a new method of determining the presence of one or more polymorphic sites in a target nucleic acid sequence. The method comprises determining changes in the melting profile of an amplified product where the amplified product was amplified with a primer pair where one of the primers is labeled, e.g., with a fluorescent label. The method employs a probe that is labeled with a label that interacts with the label on the primer, e.g., a quencher that quenches the fluorescent label on the primer, such that the signal is quenched when the probe and primer are in close proximity. A change in the melting profile of an amplified product in comparison to a control reflects the presence of the polymorphic site.
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申请公布号 |
WO2007018734(A3) |
申请公布日期 |
2009.04.16 |
申请号 |
WO2006US22983 |
申请日期 |
2006.06.13 |
申请人 |
BIO-RAD LABORATORIES, INC.;BRENEMAN, JOHN W.;MCKINNEY, NANCY |
发明人 |
BRENEMAN, JOHN W.;MCKINNEY, NANCY |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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