The present invention is to provide a method for obtaining an oligonucleotide such as RNA aptamer having high binding capacity to a target substance with easy-to-use and high purity. The method for obtaining oligonucleotide according to the present invention is as follows: A method for obtaining oligonucleotide comprising the steps of;
performing an electrophoresis of a nucleic acid molecule/target substance complex comprising a nucleic acid molecule and a target substance;
recovering said nucleic acid molecule/target substance complex;
extracting the nucleic acid molecule from said nucleic acid molecule/target substance complex;
gene amplifying said nucleic acid molecule.
申请公布号
EP2048246(A1)
申请公布日期
2009.04.15
申请号
EP20070790559
申请日期
2007.07.10
申请人
NEC SOFT, LTD.;NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE