发明名称 |
Production of clavulanic acid by genetic engineering of Streptomyces clavuligerus |
摘要 |
Genetically engineered Streptomyces clavuligerus strains with improved capabilities to produce clavulanic acid are provided. The strains are genetically engineered by disrupting newly identified glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes. This results in an increased intracelluar pool of the clavulanic acid precursor D-glyceraldehyde-3-phosphate (D-G3P), and increased clavulanic acid production. Clavulanic acid production may be further increased by supplying arginine to the medium in which the S. clavuligerus is grown.
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申请公布号 |
US7517680(B2) |
申请公布日期 |
2009.04.14 |
申请号 |
US20060530118 |
申请日期 |
2006.09.08 |
申请人 |
JOHNS HOPKINS UNIVERSITY |
发明人 |
TOWNSEND CRAIG A.;LI RONG-FENG |
分类号 |
C12N1/21;C07H21/04;C12P17/00 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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