METHOD OF OBTAINING TRANSGENIC CORN PLANTS

摘要

FIELD: genetic engineering. ^ SUBSTANCE: invention can be used in corn selection in creating new sorts and hybrids by means of genetic engendering, in works on insertional mutagenesis, separating and cloning of corn genes. Generative organs of corn are processed with suspension of cells of strain Agrobacterium tumefaciens with activated vir-genes. As generative organs, blooming female gametophyte is used. Before processing said suspension is mixed with substances inert with respect to strain cells and characterised by osmotic potential exceeding osmotic potential of suspension for obtaining isotonic solution. As such substances sucrose and/or glycerin are used. After procession with suspension generative organs are pollinated with pollen of fertile plants. In gametophyte pistil filaments are used, which are cut at height of 1-2 cm from corncob before processing with suspension. Processing is carried out by means of pipette into the depth of cut part of pistil filaments. Pollination is performed with pollen preliminary collected into packet, which is applied onto cut of pistil filaments with fingers or shaken onto it. ^ EFFECT: method allows to reduce term of obtaining transgenic corn plant and increase yield of final product, preserving high obtaining frequency. ^ 5 cl, 1 dwg