ENZYMATIC SYNTHESIS OF SULFATED POLYSACCHARIDES WITHOUT IDURONIC ACID RESIDUES

摘要

lduronic acid (IdoUA)-free heparan sulfate (HS)-like compounds are provided. Also provided are methods of synthesizing IdoUA-free HS-like compounds. The methods can include providing at least one O- sulfotransferase (OST) enzyme and a reaction mixture comprising 3'- phosphoadenosine 5'-phosphosulfate (PAPS); and incubating a polysaccharide substrate with the at least one OST and the reaction mixture, whereby the HS- like compounds are synthesized. Also disclosed are methods of synthesizing a library of HS-like compounds and methods of determining the mechanism of activity of HS-like compounds.