HIGH PRESSURE TREATMENT OF AGGREGATED INTERFERONS

摘要

High pressure to treat aggregated interferons, particularly recombinant human interferon-ß, to reduce the aggregate content of interferon material. Highly 5 pure, soluble monomelic recombinant interferon-ß is prepared in representative embodiments. Multiple strategies may be used in combination that make nonglycosylated IFN-ß more amenable to high pressure treatment. It has been found that refolding yields of high pressure treatment can be significantly improved by use of a combination of strategies, including, or example a pre-treatment of the 10 IFN-ß that involves solubilizing and then precipitating the protein. This pretreatment is particularly effective with respect to recombinant IFN-ß inclusion bodies recovered from host cells such as E. coli cells.