MICROSCOPE AND METHOD FOR OPERATING A MICROSCOPE

摘要

The invention relates to a method for operating a microscope, wherein excitation light is focused or radiated onto or into different points of a sample, wherein an intensity of the excitation light is varied point-specifically, and wherein an intensity of light reflected from the sample is measured in a point-specific and quantitative manner in at least one spectral region. According to the invention, the method is characterized in that the intensity and/or a spectral composition of the excitation light radiated into a defined point of the sample is automatically adjusted by way of a control device, as a function of information previously provided from measurement data of the sample about an anticipated or actual intensity of the light reflected from said point in the spectral region, such that an integral of the intensity of the light reflected from said point in the spectral region over a pixel dwell time is within a value interval to be determined. The invention further relates to a microscope.