PRIMERS FOR PCR AMPLIFICATION COMPRISING ABASIC PARTS WITHIN THE PRIMER SEQUENCES

摘要

A primer for PCR amplification comprising abasic parts within a primer sequences is provided to amplify different templates having mutational site in common by including the abasic parts without having specific coding information. A primer for PCR amplification includes abasic parts of 1-10 pieces. The abasic parts correspond to mutation base sites or multi-formation base sites of to be mixed nucleic acid template. A composition for PCR amplification includes the primer, a buffer solution for reaction, dNTP of four kinds, and a DNA polymerase. A method for PCR amplification comprises a step for mixing nucleic acid template on a compound for PCR amplification and a step for performing PCR amplification on the compound.