ATTENUATED INVASIVE E. COLI STRAINS AND APPLICATIONS THEREOF AS INTRACELLULAR VECTOR FOR THERAPEUTIC MOLECULE

摘要

The invention relates to a vectorial system capable of delivering a nucleic acid of interest into eukaryotic target cells, said vectorial system including a recombinant non-pathogenic and non-replicative Escherichia coli bacterium (E. coli) having integrated into its chromosome, in a targeted manner and without any antibiotic marker, one or more genes imparting to said E. coli bacteria the capacity to penetrate into the cytoplasm of said eukaryotic target cells and to lyse the penetration vacuole. The present invention also relates to the use of such E. coli bacteria for the production of therapeutic compositions and their delivery without any purification step for preventing or treating diseases by vaccination or gene therapy.