发明名称 |
METHODS FOR INCREASING EXPRESSION OF GENES IN A FUNGAL CELL |
摘要 |
<p>The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first polynucleotide comprising a nucleic acid sequence encoding the polypeptide operably linked to a copper-inducible promoter sequence comprising a copper-responsive upstream activation sequence activated by a copper-dependent trans-acting transcription factor and a second polynucleotide comprising one or more (several) additional copper-responsive upstream activation sequences operably linked upstream to the promoter sequence, wherein the promoter sequence is foreign to the nucleic acid sequence encoding the polypeptide and the copper-responsive upstream activation sequences are responsible for copper-induced transcription of the promoter sequence, and a third polynucleotide comprising at least one copy of a gene encoding the copper-dependent trans-acting transcription factor; and (b) isolating the polypeptide from the cultivation medium.</p> |
申请公布号 |
EP2044203(A2) |
申请公布日期 |
2009.04.08 |
申请号 |
EP20070799579 |
申请日期 |
2007.07.13 |
申请人 |
NOVOZYMES, INC.;NOVOZYMES A/S |
发明人 |
YAVER, DEBBIE;CHERRY, BARBARA;BJOERNVAD, MADS ESKELUND |
分类号 |
C12N15/81 |
主分类号 |
C12N15/81 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|