A PROCESS FOR THE ENZYMATIC REDUCTION OF ENOATES

摘要

A process for the enzymatic reduction of an enoate (1) wherein the C=C bond of the enoate (1) is stereoselectively hydrogenated in the presence of an enoate-reductase and an oxidizable co-substrate (2) in a system which is free of NAD(P)H, a. formula (1); b. in which c. A is a ketone radical (-CRO), an aldehyde radical (-CHO), a carboxyl radical (-COOR), with R = H or optionally substituted C1-C6-alkyl radical, d. R1, R2 and R3 are independently of one another H, -O-C1-C6-alkyl, -O-W with W = a hydroxyl protecting group, C1-C6-alkyl, which can be substituted, C2-C6-alkenyl, carboxyl, or an optionally substituted carbo- or heterocyclic, aromatic or nonaromatic radical, or one of R1, R2 and R3 is a -OH radical, or R1 is linked to R3 so as to become part of a 4-8-membered cycle, or R1 is linked to R so as to become part of a 4-8-membered cycle, with the proviso that R1, R2 and R3 may not be identical.