POLYPEPTIDE HAVING D-LACTATE DEHYDROGENASE ACTIVITY, POLYNUCLEOTIDE ENCODING THE POLYPEPTIDE, AND PROCESS FOR PRODUCTION OF D-LACTIC ACID

摘要

Disclosed is a transformant which is produced by introducing, into a host cell, a polynucleotide encoding any one polypeptide selected from polypeptides (A) to (C) each having a higher D-lactate dehydrogenase activity than those of conventional polypeptides so that the polypeptide can be expressed in the host cell, and which can achieve D-lactic acid fermentation with high productivity. (A) A polypeptide comprising an amino acid sequence depicted in SEQ ID NO:1 or SEQ ID NO:2. (B) A polypeptide which comprises an amino acid sequence produced by substituting, deleting, inserting and/or adding one or several amino acid residues in the amino acid sequence depicted in SEQ ID NO:1 or SEQ ID NO:2, and which has a D-lactate dehydrogenase activity. (C) A polypeptide which comprises an amino acid sequence having at least 80% sequence identity to the amino acid sequence depicted in SEQ ID NO:1 or SEQ ID NO:2, and which has a D-lactate dehydrogenase activity.