METHOD FOR THE LONG-TERM PRESERVATION OF TRANSGENIC PLANT CELLS

摘要

PURPOSE: A method for preserving transgenic plant cells is provided to maximize cell viability and to suppress cell contamination. CONSTITUTION: A method for preserving transgenic plant, tissue or cells under anaerobic condition comprises: a step of transforming a plant with a recombinant vector; a step of preparing a medium for plant cells, containing 1-300mM of sugar; and a step of preserving plant cells in the medium for a long time. The medium for plant cells is AA(Amino acid) medium, MS(Murashige and Skoog), N6, WHITE or SH(Schenk and Hiderbrandt). The promoter operated in case of sugar depletion and is an alpha-amylase RAmy3D promoter.