METHODS FOR PRODUCTION OF KNOCK-IN SOMATIC CELLS OF DAF GENE IN ALPHA 1,3-GALACTOSYLTRANSFERASE GENE LOCUS

摘要

PURPOSE: An alpha 1,3-galactosyltransferase gene targeting vector and a knock-out animal transformed by the same are provided to produce miniature pig in which DAF is expressed. CONSTITUTION: An alpha 1,3-galactosyltransferase gene targeting vector comprises: a nuclear localization signal region, a first region having 4-6kb nucleic acid sequence which is homologous with alpha 1,3-galactosyltransferase nucleic acid sequence; a gene region encoding DAF(decay accelerating factor); a positive selection marker region, and a second region having 2-4 kb nucleic acid sequence which is homologous with alpha 1,3-galactosyltransferase.