发明名称 |
Nucleic acid detection assay |
摘要 |
A method for determining the methylation status of a potential methylation site in genomic nucleic acid comprising treating genomic nucleic acid with an agent which modifies cytosine bases but does not modify 5methyl-cytosine bases under conditions to form a. modified nucleic acid template containing a potential methylation site; providing a first clamp containing a first capture sequence complementary to a region flanking one side of the potential methylation site in the modified nucleic acid template, providing a second clamp containing a second capture sequence complementary to a region flanking the other side of the potential methylation site in the modified nucleic acid template; allowing the first clamp and the second clamp to hybridise to the modified nucleic acid template; ligating the hybridised first and second clamps to form a probe spanning the potential methylation site in the modified nucleic acid template; digesting the modified nucleic acid template to obtain the probe; and detecting the probe and determining the methylation status of the potential methylation site in the modified genomic nucleic acid.
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申请公布号 |
US7846693(B2) |
申请公布日期 |
2010.12.07 |
申请号 |
US20040570715 |
申请日期 |
2004.09.03 |
申请人 |
HUMAN GENETIC SIGNATURES PTY. LTD. |
发明人 |
MILLAR DOUGLAS SPENCER;MELKI JOHN ROBERT;GABOR MIKLOS GEORGE L. |
分类号 |
C12P19/34;C12Q1/68 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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