method for revealing of RNA of trout hemorrhagic septicemia virus By means of polymerase chain reaction

摘要

A method for revealing of RNA of trout hemorrhagic septicemia virus by means of polymerase chain reaction comprises the revealing in studied probes of specific fragments of nucleic acid (RNA) by means of reverse transcriptase polymerase chain reaction. For reverse transcriptase polymerase chain reaction the artificially synthesized oligonucleotide primers having the following nucleotide chain:NO- - 5'-TGTCYGTKCTTCTCTCYTATG - 3'NO-7R - 5'-ATCATCTTKTCCACMGAG - 3',where: Y=C/T, M=A/C, K=G/T.