发明名称 |
METHOD OF PRODUCING HIGH-POLYMERIC RNA FROM DRY BAKER'S YEAST |
摘要 |
FIELD: chemistry; biochemistry. ^ SUBSTANCE: invention relates to biotechnology, in particular to extraction of physiologically active compounds and can be used in medicine. Dry yeast is suspended in a 1-2% aqueous solution of oleic acid titrated with an alkali to pH 7-8. The suspension is kept at 99-102C for 40-60 minutes. Lysate cooled to 35-45C is centrifuged without cooling. The supernatant fluid is discharged and NaCl is added to the discharged fluid until achieving concentration of 2-3 M. The suspension is kept for 20-24 hours at room temperature and centrifuged without cooling. Low-polymeric RNA is extracted from the obtained supernatant and the precipitate-extraction cake is washed with two portions of 2-3 M solution of NaCl and with four portions of 92-97% ethanol via successive resuspension at room temperature and centrifuging. High-polymeric RNA is extracted from the obtained extraction cake using distilled water and then dried using a conventional method. Ethanol is reclaimed through distillation. Natural oligoribonucleotides are extracted from the low-polymeric RNA through gel filtration on Sepharose-4B. ^ EFFECT: invention enables to obtain high-polymeric RNA with considerably high molecular weight as a result of using dry baker's yeast without preliminary soaking or using high temperature. ^ 5 cl, 1 dwg, 1 ex |
申请公布号 |
RU2403288(C1) |
申请公布日期 |
2010.11.10 |
申请号 |
RU20090128847 |
申请日期 |
2009.07.27 |
申请人 |
OBSHCHESTVO S OGRANICHENNOJ OTVETSTVENNOST'JU "VITALANG" |
发明人 |
JAMKOVAJA TAT'JANA VITAL'EVNA;ZAGREBEL'NYJ STANISLAV NIKOLAEVICH;PANIN LEV EVGEN'EVICH;JAMKOVOJ VITALIJ IVANOVICH |
分类号 |
C12N1/18;C12N15/10;C12P19/34 |
主分类号 |
C12N1/18 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|