RHODOCOCCUS RHODOCHROUS NCIMB 41164 STRAIN AND USE THEREOF AS NITRILE HYDRASE PRODUCER

摘要

FIELD: chemistry; biochemistry. ^ SUBSTANCE: invention relates to biotechnology. The Rhodococcus rhodochrous NCIMB 41164 strain produces nitrile hydrase. The strain is cultured in a culture medium containing urea or a urea derivative. Urea or derivative there of is added to the culture medium at least 6 hours after the microorganism begins to grow. Biocatalytic activity of the Rhodococcus rhodochrous NCIMB 41164 strain increases and is equal to 250-300000 mcmol/min/g of the dry biomass of cells. Nitrile hydrase obtained from said strain can convert (meth)acrylonitrile to (meth)acrylamide. The invention also discloses methods of producing amide from the corresponding nitrile. The methods involve reaction of nitrile hydrase in an aqueous medium in the presence of a biocatalyst which is a Rhodococcus rhodochrous NCIMB 41164 strain and the amide is (meth)acrylamide. The invention also discloses a suspension for storing biocatalyst, containing inactively growing cells of this strain, water and optionally residual components of the fermentation broth. The biocatalyst is stored at temperature higher than freezing point, preferably higher than 0C and specifically from 4 to 30C. ^ EFFECT: higher biocatalytic activity of the Rhodococcus rhodochrous NCIMB 41164 strain. ^ 23 cl, 5 tbl, 7 ex