COMBINATORIAL DNA LIBRARY FOR PRODUCING MODIFIED N-GLYCAN IN LOWER EUKARYOTE

摘要

PROBLEM TO BE SOLVED: To provide an engineered eukaryotic host cell which can be used to express and target any desirable gene involved in glycosylation for producing a therapeutic glycoprotein for mammalian (e.g. human).

SOLUTION: There are provided a method for producing a human-like glycoprotein in a non-human eukaryotic host cell having an oligosaccharide modified by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases; and nucleic acid molecules and combinatorial libraries which can be used to target and express mammalian enzymatic activity such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell.

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