| 摘要 |
FIELD: medicine, veterinary science. ^ SUBSTANCE: group of inventions relates to the field of veterinary microbiology. Method for production of vaccine includes extraction of lymphoid cells infected with merocytes Theileria annulata from an animal infected with merocytes Theileria annulata in the form of supernatant from ground mites or in the form of preserved culture, cultivation of infected cells is carried out in growth medium, which represents RPMI 1640 complemented by serum in amount from 10 to 20 % selected from embryonal serum of calf, newborn calf serum or normal bovine serum; solution of antibiotics containing sodium benzylpenicillin 100 ME per ml, sulfate of streptomycin 100 microgram per ml and mycostatin 10 ME per ml; and also 200 mM L-glutamin per ml, at the same time all components are previously sterilised by filtration and purified from any contamination by microorganisms by standard methods. Reproduction of cultivated cells in vitro is done by method of serial passages, at the same time up to 100 passages are carried out with periodicity of two or three times per week, and density of cells inoculation is maintained accordingly as 1ù105 or 2ù105 cells per ml, or by method of their limit dilution in "cupark" trays and growing cloned cultures from one cell by cultures passage up to 200 times in mode of two/three times per week. Cultures are grown up to large volumes to produce vaccine. Vaccine efficiency is tested in wide field conditions on cows Bos Taurus mated with cows Bos Indicusy, and also on newborn calves, less than two months old calves and impregnate cows. Vaccine is safe for all age groups of cattle, including newborn calves and impregnate cows, has high immunogenic activity. ^ EFFECT: development of safe vaccine against theileriosis. ^ 8 cl, 1 tbl, 1 ex |
