发明名称 |
MUTANT SepRS, AND METHOD FOR SITE-SPECIFIC INTRODUCTION OF PHOSPHOSERINE INTO PROTEIN BY USING THE SAME |
摘要 |
A mutant SepRS which is suitable for a site-specific introduction of phosphoserine into a protein is prepared by analyzing the structure and functions of a phosphoseryl-tRNA synthetase (SepRS) derived from an archaebacterium. A mutant SepRS composed of an amino acid sequence depicted in SEQ ID NO:2, in which any one or more of glutamic acids at position-418 and position-420 and threonine at position-423 are substituted with other amino acid, and having enhanced binding affinity with a suppressor tRNA as compared with a wild type phosphoseryl-tRNA synthetase (SepRS) composed of an amino acid sequence depicted in SEQ ID NO:2 is provided. |
申请公布号 |
EP2048233(B1) |
申请公布日期 |
2011.11.16 |
申请号 |
EP20070768165 |
申请日期 |
2007.06.28 |
申请人 |
RIKEN;THE UNIVERSITY OF TOKYO |
发明人 |
YOKOYAMA, SHIGEYUKI;FUKUNAGA, RYUYA;SEKINE, SHUN-ICHI |
分类号 |
C12N15/09;C12N9/00;C12P21/02 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|