发明名称 |
SYNTHESIS OF FLUORESCENT NOBLE METAL NANOPARTICLES |
摘要 |
A process for the production of fluorescent nanoparticles selected from noble metal, silica or polymer nanoparticles which comprises: 1. A process for the production of fluorescent nanoparticles selected from noble metal or silica nanoparticles which comprises: (1) providing a platform of nanoparticles; (2) covering the surfaces of the nanoparticles to saturation with thiol-terminated polymers by one of the following methods: 1. mixing the nanoparticles with methoxy-(polyethylene glycol)-thiol and biotin-(polyethylene glycol)- thiol; 2. mixing the nanoparticles with fluorescently-labeled methoxy-(polyethylene glycol)-thiol and/or biotin-(polyethylene glycol)-thiol 3. coordinating thiol and biotin thiol to the surfaces of the nanoparticles by a non-covalent bond; and 4. directly conjugating methoxy-thiol and biotin-thiol to the surfaces of the nanoparticles, so that the polymers bind to the surfaces of the nanoparticles as a brush layer via thiol particle coordination of the thiol ends so that the biotin or methoxy ends are free; and (3) homogeneously mixing the resulting biotin nanoparticles with fluorescent avidin or a derivative thereof in proportions such that the final concentration is 1 biotin molecule for every 10 to 1000 avidin molecules in the fluorescent multi-coloured nanoparticle-avidin complexes, each being capable of having a different targeting molecule, and which may be mixed with biotin related targets, and the fluorescent labeled avidin or a derivative thereof being spaced away from the particle surface, thus reducing or removing the potential quenching of the dye. |
申请公布号 |
WO2012037667(A1) |
申请公布日期 |
2012.03.29 |
申请号 |
WO2011CA01080 |
申请日期 |
2011.09.23 |
申请人 |
THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO;CHAN, WARREN;PERRAULT, STEVEN;CHOU, LEO |
发明人 |
CHAN, WARREN;PERRAULT, STEVEN;CHOU, LEO |
分类号 |
C09K11/02;A61K9/14;B01J2/00;B22F1/00;G01N33/53;G01N33/58 |
主分类号 |
C09K11/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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