This invention relates to a method of producing a recombinant enzyme, more particularly, this invention relates to a method of producing water soluble enzymatically active recombinant glycine N-acyltransferase (GLYAT (E.G. 2.1.3.13)), including the steps of providing a suitable expression host; preparing a vector including a gene for expressing GLYAT in the expression host to form an expression piasmid; transforming the host with the expression piasmid to form an expression system; expressing the GLYAT gene in the expression system; and separating the expressed GLYAT from the expression system.
申请公布号
WO2012025895(A3)
申请公布日期
2012.05.03
申请号
WO2011IB53721
申请日期
2011.08.24
申请人
NORTH-WEST UNIVERSITY;MIENIE, LODEWYK, JACOBUS;VAN DIJK, ALBERDINA, AIKE;BADENHORST, CHRISTOFFEL, PETRUS, STEPHANUS
发明人
MIENIE, LODEWYK, JACOBUS;VAN DIJK, ALBERDINA, AIKE;BADENHORST, CHRISTOFFEL, PETRUS, STEPHANUS