METHOD FOR TRANSFERRING EMBRYO IN COW USING SOLUTION FOR EMBRYO TRANSFER COMPRISING TOPICAL ANTI-INFLAMMATORY AGENT

摘要

PURPOSE: A method for transferring a fertilized egg of cows is provided to improve pregnancy rate in cows by suppressing an inflammatory reaction with dexamethasone and rhLIF, thereby increasing productivity of Korean beef cattle. CONSTITUTION: A method for transferring a fertilized egg of cows comprises the following steps: (a) externally aging a cow egg, externally fertilizing the egg with sperm, and externally culturing the fertilized egg; (b) putting the fertilized egg in a transferring solution containing dexamethasone and a recombinant human leukemia inhibitory factor(rhLIF), and loading the fertilized egg and the solution in straw; and (c) directly injecting the straw loaded with the fertilized egg and the solution, and transferring the fertilized egg. [Reference numerals] (1) Verifying based on the occurrence of re-estrus between 23 days and 42 days; (2) Analyzing pregnancy when 60 days after the pregnancy; (AA) Solution for transferring a fertilized egg; (BB) Basic solution(TCM-199 + 10% FBS); (CC) 100nM/ml Dexamethasone and 100nM/ml rhLIF; (DD) Washing twice to three times; (EE) Washing using the same solution; (FF) Loading a straw; (GG) Loading a fertilized egg and the solution for transferring a fertilized egg in the straw; (HH) Transferring the fertilized egg; (II) Analyzing pregnancy;