| 摘要 |
PROBLEM TO BE SOLVED: To provide compositions and methods for increasing the longevity of a cell culture and permitting the increased production of proteins, preferably recombinant proteins, such as antibodies, peptides, enzymes, growth factors, interleukins, interferons, hormones, and vaccines.SOLUTION: There is provided a mammalian cell line in which a first nucleic acid encoding the mutant Bcl-2 protein (Bcl-EEE) is transfected to produce Bcl-EEE host cells expressing the Bcl-EEE having amino-acid substitutions of T69E, S70E and S87E, wherein the Bcl-EEE host cells are pre-adapted to proliferate and grow in serum-free medium, and in order to produce the cell line transfected and pre-adapted, the pre-adapted Bcl-EEE host cells allow a second nucleic acid encoding mutant proteins to be further transfected in the serum-free medium. |
