Methods and Compositions for Analysis of Nucleic Acids

摘要

Compositions and methods for analysis of nucleic acids are disclosed. Targets are hybridized to arrays having features that include pairs of co-localized probes within features. The probe pairs may include a first probe type that is oriented so that the 5' end is free and the 3' end is attached to the support and a second probe type that is oriented so that the 3' end is free for extension and the 5' end is attached to the support. The probes of a feature are complementary to different regions of the same target sequence so they can simultaneously hybridize to a single target with a gap or nick between. The gap may be filled by extension and ligation or ligation.