GENOME SURGERY WITH PAIRED, PERMEANT ENDONUCLEASE EXCISION
摘要
<p>The use of P2E2 constructs in genome surgery includes a cell penetration component, a DNA binding component and a restriction endonuclease. The method for performing genome surgery includes: a) providing one or more recombinant of the P2E2 constructs; b) penetrating a cell with the recombinant P2E2 protein construct; c) forming a protein product in the cell by the processes of transcription and translation or by direct introduction of the P2E2 protein construct to the cell; d) attaching the protein product of the P2E2 construct to one or more targeted genomic sequences within the cell; and e) the endonuclease of the P2E2 construct cutting both strands of the genome at target locations.</p>
申请公布号
WO2014011817(A2)
申请公布日期
2014.01.16
申请号
WO2013US49987
申请日期
2013.07.10
申请人
THE BOARD OF REGENTS OF NEVADA SYSTEM OF HIGHER EDUCATION ON BEHALF OF THE UNIVERSITY OF NEVADA, LAS VEGAS;SCHILLER, MARTIN;STRONG, CHRISTY