Development of the primer set for loop-mediated isothermal amplification to detect Azumiobodo hoyamushi in soft tunic syndrome of the edible ascidian, Halocynthia roretzi

摘要

The present invention relates to an inspecting kit and an inspecting method using a probe and a primer for inspecting Azumiobodo hoyamushi which are pathogenic protozoa causing a soft tunic syndrome in Halocynthia roretzi. The present invention relates to an inspecting kit (oligonucleotide microarray) and an inspecting method for rapidly detecting a pathogenic organism of a soft tunic syndrome in Halocynthia roretzi by producing a primer through a probe which is specific to a PCR amplification condition in which a 18S rDNA gene of Azumiobodo hoyamushi causing soft tunic syndrome in Halocynthia roretzi can be identified. Consequently, the Halocynthia roretzi of Azumiobodo hoyamushi which is known as the cause of soft tunic syndrome in Halocynthia roretzi is rapidly detected from seawater or Halocynthia roretzi from the initial symptoms of soft tunic syndrome, so that the inspecting kit and inspecting method are applied to an Azumiobodo hoyamushi soft tunic syndrome forecasting system, and damage can be preemptively prevented.