COMPOSITIONS AND METHODS FOR SAMPLE PROCESSING

摘要

This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

主权项

1. A method of barcoding sample materials, comprising:
providing a first partition comprising a plurality of nucleic acid barcode molecules associated therewith, the plurality of nucleic acid barcode molecules comprising the same nucleic acid barcode sequence; co-partitioning the first partition with components of a sample material into a second partition; releasing the barcode molecules from the first partition into the second partition; and attaching the barcode molecules to one or more of the components of the sample material or fragments thereof within the second partition.