COMPOSITIONS AND METHODS FOR ANALYZING HISTIDINE PHOSPHORYLATION

摘要

A peptide is disclosed of the general structure: Z—W—Y, wherein Z and Y are independently a one to eight amino acid sequence wherein the amino acids are selected from glycine and alanine and W is a non-hydrolyzable pHis analogue. Such peptides can be used to produce sequence-independent anti-phosphohistidine antibodies. Also provided are antibodies that specifically bind to a peptide comprising a phosphohistidine (or a non-hydrolyzable pHis analogue) but fail to specifically bind to an identical peptide containing histidine instead of phosphohistidine.

主权项

1. A monoclonal antibody that specifically binds to a peptide or protein comprising a phosphorylated histidine independent of amino acid sequence, but does not specifically bind to a peptide lacking a phosphorylated histidine.