TWO DIMENSIONAL NANOFLUIDIC CCD ARRAYS FOR MANIPULATION OF CHARGED MOLECULES IN SOLUTION

摘要

Methods and apparatus for manipulation of charged molecules in solution. More particularly, the invention provides nanofluidic CCD arrays that are capable of manipulate one or a group of molecules on an individual bases such that they undergo controlled physical and/or chemical movements and/or transformations. The nanofluidic apparatus and methods that are capable of individualized movement and controlled manipulation of a molecule or group of molecules in solution environment. A key feature of the nanofluidic CCD array technology disclosed herein is that molecules or nanoparticles, in particular DNA and other large biomolecules, can be individually manipulated and moved to spatially distinctive parts of the nanofluidic device, without the use of electrophoresis or pressure. A nanofluidic CCD array can be constructed such that different regions of the array have different biochemical activities. Different DNA molecules in a single nanofluidic device can be addressed and treated individually, all without the use of valves.