METHOD FOR GUIDING THE DERIVATION OF ENDOTHELIAL CELLS FROM HUMAN PLURIPOTENT STEM CELLS EMPLOYING TWO-DIMENSIONAL, FEEDER-FREE DIFFERENTIATION

摘要

This invention relates, e.g., to a method for differentiating mammalian (e.g., human) pluripotent stem cells (PSCs) into endothelial cells (ECs) in vitro, by plating a single-cell suspension of PSCs onto a suitable surface such as type IV collagen and culturing the cells with VEGF after which ECs can be harvested. A preferred embodiment of the method first cultures the cells without VEGF and then sequentially cultures the cells with VEGF. Differentiation can be enhanced by adding an inhibitor of transforming growth factor β to the culturing with VEGF.

主权项

1. A method for differentiating mammalian pluripotent stem cells (PSCs) into endothelial cells (ECs) in vitro, comprising the steps of:
plating a single-cell suspension of PSCs onto a suitable surface; culturing the cells in the presence of VEGF; and harvesting the cultured cells whereby ECs are produced.