CELL CULTURE SYSTEMS

摘要

An integrated cell culture system may include one or more cell culture vessels, manipulation apparatus, pumping apparatus, cell release apparatus, monitoring apparatus, and a control apparatus. The control apparatus may be used to monitor and control the system to facilitate effective cell culturing. The cell release apparatus may be used to release a plurality of cells adhered to the cell culture surfaces of the cell culture vessels.

主权项

1. A cell culture system comprising:
at least one cell culture vessel configured to culture cells using a plurality of parallel cell culture surfaces, wherein the at least one cell culture vessel comprises at least one port configured to allow material to flow into and out of the at least one cell culture vessel; manipulation apparatus configured to rotate the at least one cell culture vessel about a first rotation axis and about a second rotation axis, wherein the first rotation axis is perpendicular to the second rotation axis, wherein each of the first rotation axis and the second rotation axis are parallel a ground surface; pumping apparatus fluidly coupled to the at least one port of the at least one cell culture vessel and configured to pump material into and out of the at least one cell culture vessel through the at least one port; monitoring apparatus configured to monitor one or more parameters of the at least one cell culture vessel, the manipulation apparatus, and the pumping apparatus; and control apparatus operably coupled to the manipulation apparatus, the pumping apparatus, and the monitoring apparatus, wherein the control apparatus is configured to coordinate movement of the at least one cell culture vessel using the manipulation apparatus with the pumping of material into and out of the at least one culture vessel using the pumping apparatus.