A platform for preparing a sequence defined biopolymer in vitro is disclosed. The platform includes a ribosome-depleted cellular extract ribosomal RNAs prepared by in vitro transcription and purified ribosomal proteins depleted of ribosomal RNAs. A method of synthesizing and assembling ribosomes in vitro for use in the platform is provided, as well as a method for preparing a sequence defined biopolymer in vitro using assembling ribosomes and the platform.
申请公布号
WO2014176469(A3)
申请公布日期
2015.01.29
申请号
WO2014US35376
申请日期
2014.04.24
申请人
NORTHWESTERN UNIVERSITY
发明人
JEWETT, MICHAEL, CHRISTOPHER;FRITZ, BRIAN, ROBERT;TIMMERMAN, LAURA, ELYSE;LIU, YI