LENTIVIRAL VECTORS AND USES THEREOF

摘要

The invention relates to compositions containing polynucleotide vectors capable of expressing a nucleic acid encoding a fusion polypeptide on the surface of a viral particle and/or a eukaryotic cell.

主权项

1. A method of displaying a candidate polypeptide on the surface of a mammalian cell, comprising the steps of:
a) contacting a packaging cell with a viral display vector comprising a nucleic acid sequence encoding a fusion protein under conditions such that said packaging cell generates one or more viral particles comprising said nucleic acid sequence, wherein said fusion protein comprises:
i) a leader peptide;ii) a candidate polypeptide;iii) at least a portion of an immunoglobulin molecule;iv) a transmembrane moiety; and b) contacting said viral particles with a mammalian cell, under conditions sufficient to express said fusion protein in said mammalian cell, wherein said candidate polypeptide is displayed on the surface of the mammalian cell.