| 主权项 |
1. A method of evaluating an inhibitory effect on damp-dry malodor, comprising the steps of:
bringing microorganisms which produce damp-dry malodor-causing substances and a test substance into contact with each other in the presence of a sebaceous dirt component; detecting expression of at least one gene selected from the group consisting of a fatty acid desaturase gene and a β oxidation-related enzyme gene derived from the microorganisms; and thereby evaluating a damp-dry malodor inhibitory function of the test substance based on a change in expression amount of the at least one gene; wherein the expression of the at least one gene is detected by using at least one oligonucleotide pair selected from the group consisting of: an oligonucleotide pair containing oligonucleotides (a) and (b) as described below, an oligonucleotide pair containing oligonucleotides (c) and (d) as described below, an oligonucleotide pair containing oligonucleotides (e) and (f) as described below, an oligonucleotide pair containing oligonucleotides (g) and (h) as described below, an oligonucleotide pair containing oligonucleotides (i) and (j) as described below, an oligonucleotide pair containing oligonucleotides (k) and (l) as described below, an oligonucleotide pair containing oligonucleotides (m) and (n) as described below, an oligonucleotide pair containing oligonucleotides (o) and (p) as described below, an oligonucleotide pair containing oligonucleotides (q) and (r) as described below, an oligonucleotide pair containing oligonucleotides (s) and (t) as described below, an oligonucleotide pair containing oligonucleotides (u) and (v) as described below, an oligonucleotide pair containing oligonucleotides (w) and (x) as described below, an oligonucleotide pair containing oligonucleotides (y) and (z) as described below, an oligonucleotide pair containing oligonucleotides (a1) and (b1) as described below, and an oligonucleotide pair containing oligonucleotides (c1) and (d1) as described below: wherein oligonucleotide (a) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 13, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 13 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene; oligonucleotide (b) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 14, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 14 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene; oligonucleotide (c) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 15, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 15 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene; oligonucleotide (d) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 16, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 16 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene; oligonucleotide (e) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 17, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 17 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene; oligonucleotide (f) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 18, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 18 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene; oligonucleotide (g) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 19, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 19 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene; oligonucleotide (h) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 20, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 20 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene; oligonucleotide (i) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 21, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 21 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene; oligonucleotide (j) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 22, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 22 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene; oligonucleotide (k) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 23, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 23 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene; oligonucleotide (1) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 24, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 24 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene; oligonucleotide (m) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 25, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 25 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene; oligonucleotide (n) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 26, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 26 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene; oligonucleotide (o) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 27, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 27 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene; oligonucleotide (p) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 28, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 28 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene; oligonucleotide (q) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 29, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 29 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene; oligonucleotide (r) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 30, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 30 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene; oligonucleotide (s) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 31, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 31 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene; oligonucleotide (t) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 32, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 32 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene; oligonucleotide (u) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 33, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 33 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene; oligonucleotide (v) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 34, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 34 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene; oligonucleotide (w) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 35, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 35 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene; oligonucleotide (x) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 36, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 36 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene; oligonucleotide (y) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 37, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 37 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the oxidation-related enzyme gene; oligonucleotide (z) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 38, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 38 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene; oligonucleotide (a1) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 39, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 39 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene; oligonucleotide (b1) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 40, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 40 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene; oligonucleotide (c1) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 41, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 41 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene; and oligonucleotide (d1) is an oligonucleotide having the base sequence set forth in SEQ ID NO: 42, or an oligonucleotide having a base sequence set forth in SEQ ID NO: 42 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene. |
