Use of active cytokinin synthase gene

摘要

Cytokinin is a plant hormone that regulates various physiological activities including cell division, cell cycle, cellular senescence, and axillary buds outgrowth. The present invention provides a method for producing a transformed plant by introducing an isolated nucleic acid or recombinant vector containing the isolated nucleic acid into a plant cell such that the amount of active cytokinin synthesized from nucleotide cytokinin is increased in the plant cell and by regenerating a plant body from the plant cell.

主权项

1. A method for producing a transformed plant, the method comprising
introducing an isolated nucleic acid or recombinant vector comprising said isolated nucleic acid into a plant cell such that the amount of active cytokinin synthesized from nucleotide cytokinin is increased in the plant cell, and regenerating a plant body from the plant cell, wherein the isolated nucleic acid is: (a) an isolated nucleic acid consisting of SEQ ID NO:1; (b) an isolated nucleic acid consisting of a sequence which hybridizes under stringent conditions to SEQ ID NO:1 and encodes a protein having activity of catalyzing reactions that synthesize active cytokinin from nucleotide cytokinin, wherein the stringent conditions comprise washing in 15 mM to 600 mM sodium salt at 50° C. to 70° C.; (c) an isolated nucleic acid encoding a protein consisting of the amino acid sequence of SEQ ID NO:2; or (d) an isolated nucleic acid encoding a protein which consists of an amino acid sequence having 72.2% or higher homology to the amino acid sequence of SEQ ID NO:2 and that has activity of catalyzing reactions that synthesize active cytokinin from nucleotide cytokinin.