| 摘要 |
<p>A method of measuring a sample (3) comprises the steps of (i) providing light of a first composition; (ii) selecting a particle (2) from a group of particles which are driven to emit photons when subjected to the light (4) of the first composition; (iii) forming the light of the first composition to provide a light intensity distribution comprising a spatially limited minimum (19); (iv) applying the light intensity distribution to the sample such that the particle is located in the spatially limited minimum of the light intensity distribution; (v) detecting the photons emitted by the particle; and tracking the movement of the particle with the minimum of the light intensity distribution by (vi) moving the light intensity distribution with respect to the sample such that a rate of the photons emitted by the particle remains minimal, and (vii) taking an actual position of the minimum of the light intensity distribution in the sample as an actual position of the particle in the sample. For imaging the sample the method comprises the further steps of (viii), for each of a plurality of parts of the sample, determining a dwell time of the particle; and (ix) mapping a distribution of the dwell times over the sample.</p> |
