METHOD FOR INHIBITING NON-SPECIFIC BINDING IN STEP OF DETECTING SUBSTANCE IN BIOLOGICAL SAMPLE, AND AGENT FOR USE IN THE METHOD

摘要

The present invention provides a method of inhibiting non-specific binding in a step of detecting a substance in a biological sample. The invention provides a method of inhibiting non-specific binding in a step of detecting a substance in a biological sample, the method comprising:
bringing a biotin-binding protein monomer not substantially having biotin-binding ability into contact with the biological sample, wherein
in the step of detecting, a substance capable of specifically binding to the substance to be detected is immobilized to a carrier through binding between biotin and the biotin-binding protein before or after binding of the substance to be detected to the substance capable of specifically binding to the substance to be detected.