| 主权项 |
1. A method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes, the method comprising:
(a) contacting the sample with a transmembrane pore and a panel of two or more probes, wherein each probe recognises one or more of the analyte members and comprises (i) an aptamer that binds to one or more of the analyte members and (ii) a tail which is capable of entering the pore and has different effects on the current flowing through the pore depending on whether or not the aptamer in the probe is bound to one of the analyte members, wherein each probe affects the current flowing through the pore in a distinctive manner, and wherein each analyte member of the group of two or more analytes is recognised by at least one probe in the panel; and (b) measuring the current flowing through the pore to determine which probes in the panel, if any, have bound to an analyte member and thereby determining the presence or absence of one or more analyte members in the sample. |
