Compositions and methods for prevention of escape mutation in the treatment of HER2/NEU over-expressing tumors

摘要

This invention provides compositions and methods for treating and vaccinating against an Her2/neu antigen-expressing tumor and inducing an immune response against dominant and several sub-dominant epitopes of the antigen.

主权项

1. A recombinant Listeria strain having mutations in the D-alanine racemase (Dal) gene and the D-amino acid transferase (Dat) gene, said recombinant Listeria strain comprising a nucleic acid encoding a first and a second open reading frame, wherein said first open reading frame encodes a recombinant polypeptide comprising SEQ ID NO: 2 fused to a) non-hemolytic LLO protein or N-terminal fragment, b) a PEST sequence, or c) an ActA fragment, and wherein said second open reading frame encodes a metabolic enzyme that complements said mutations, and wherein said recombinant Listeria lacks the ActA virulence gene.