METHOD OF FRACTION COLLECTION FOR A LIQUID CHROMATOGRAPHY SYSTEM

摘要

Described are a fraction collector and a method of fraction collection for a liquid chromatography system. The method includes diverting a liquid chromatography system flow from a waste channel to a collection tube at a start of a fraction collection window and collecting the system flow dispensed from the collection tube during the fraction collection window. At the end of the window, the system flow is diverted to the waste channel and a flow of a wash solvent is provided to the collection tube to dispense liquid remaining in the collection tube at the end of the window from the collection tube. The method can increase the amount of the collected fraction and can reduce or eliminate cross-contamination of a subsequently collected fraction. The method is useful for analytical scale applications where the collected fractions may have volumes defined by a limited number of drops dispensed from the collection tube.

主权项

1. A fraction collector for a liquid chromatography system, the fraction collector comprising:
a valve having a first port to receive a liquid chromatography system flow, a second port in fluidic communication with a waste channel, a third port and a fourth port, the valve having a first state in which the first port is in fluidic communication with the third port and the second port is in fluidic communication with the fourth port, the valve having a second state in which the first port is in fluidic communication with the second port and the third port is in fluidic communication with the fourth port, the valve having a control port to receive a valve control signal to control the valve to be in one of the first or second states; a wash solvent source in fluidic communication with the fourth port and configured to provide a flow of a wash solvent, the wash solvent source having a control port to receive a wash control signal to control the flow of the wash solvent; and a collection tube in fluidic communication with the third port to receive the liquid chromatography system flow when the valve is in the first state and to receive the flow of wash solvent from the wash solvent source when the valve is in the second state.