| 摘要 |
FIELD: chemistry.SUBSTANCE: invention relates to biochemistry. An isolated G-CSF precursor comprising a signal peptide and a G-CSF peptide, wherein the signal peptide has the sequence of the human wild-type signal peptide of the human G-CSF/b molecule but has at least one mutation, including deletion of Glu29, substitution His21Phe, and substitution Gln28Leu. Further embodiments of the invention are a polynucleotide coding for the G-CSF precursor, expression vector, transfected cell and G-CSF isolation from the said cell culture.EFFECT: invention enables more precise processing of the G-CSF precursor protein with reduced N-terminal truncation.13 cl, 13 dwg, 10 ex |
