| 摘要 |
A nucleic acid amplification reaction device includes a rotary body (61) which has a mounting portion, on which a cartridge (1) is mountable, the cartridge (1) including a tube (20) having, in this order in the inside thereof, a first plug (44) formed of oil, a fourth plug (47) formed of an elution solution which undergoes phase separation from oil and causes nucleic acid to be eluted from nucleic acid-binding magnetic carriers bound to the nucleic acid, and a fifth plug (48) formed of oil, and a nucleic acid amplification reaction container (30) which communicates with the side of the tube (20) on which the fifth plug (48) is disposed and in which a nucleic acid amplification reaction reagent is held in oil, a heating unit (65A-65C) which heats the fourth plug (47) and has a cylindrical groove (68), with which the tube (20) is engaged when the cartridge (1) is mounted on the mounting portion, and is exposed in a slit shape through an opening (67) of the cylindrical groove (68), and a magnetic force applying mechanism (71) which applies a magnetic force to the nucleic acid-binding magnetic carriers to move the nucleic acid-binding magnetic carriers in a longitudinal direction of the tube (20). The posture of the cartridge (1) is changed by the rotation of the rotary body (61) and droplets containing the elution solution introduced from the tube (20) move inside the nucleic acid amplification reaction container (30). |
