发明名称 |
TAL-MEDIATED TRANSFER DNA INSERTION |
摘要 |
The invention relates to methods for stably integrating a desired polynucleotide into a plant genome, comprising transforming plant material with a first vector comprising nucleotide sequences encoding TAL proteins designed to recognize a target sequence; transforming the plant material with a second vector comprising (i) a marker gene that is not operably linked to a promoter (“promoter-free marker cassette”) and which comprises a sequence homologous to the target sequence; and (ii) a desired polynucleotide; and identifying transformed plant material in which the desired polynucleotide is stably integrated. |
申请公布号 |
EP2922959(A1) |
申请公布日期 |
2015.09.30 |
申请号 |
EP20130856843 |
申请日期 |
2013.11.19 |
申请人 |
J.R. SIMPLOT COMPANY |
发明人 |
ROMMENS, CAIUS M.;DUAN, HUI;WEEKS, TROY J. |
分类号 |
C12N15/82;A23L19/12;A23L19/18 |
主分类号 |
C12N15/82 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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